Yuya discovers that the man before her is no longer Kyooshiroo, but Onime-no-Kyo. Kyo defeats the Bantouji brothers easily and viciously and turns to face the last person alive: Yuya. Kyo spares her, promising to watch as she hunts for her brother's killer and to kill her if she fails. Once he puts his sword away, Kyo is gone and the spirit of Kyooshiroo returns to his body. Kyooshiroo leaves the town before the spirit of Kyo can harm his friends, but Yuya insists on traveling with him. (After all, there is a huge bounty on Kyo). As they head West, they are watched by a mysterious lady who has her own plan for Kyo ...

Kyoshiro Mibu
A traveling medicine peddler, Kyoshiro Mibu is peaceful, lighthearted, and a bit of a coward. He carries a 5 shaku long sword with him, but claims it's just for show. Unbeknownst to anyone, he holds within him the spirit of Demon Eyes Kyo, and when provoked, the red-eyed samurai takes over Kyoshiro's body.

Demon Eyes Kyo
Kyo is the other personality in Kyoshiro's body. Kyo, one of the original Muramasas, has blood red eyes and is said to have killed 1000 men at the Battle of Sekigahara. There's a legendary one million ryo bounty on his head.

Yuya Shiina
Yuya is a clever and beautiful bounty hunter, the best anywhere on Tokaido-chu. She seeks a man with a scar on his back - the man who killed her brother. After discovering the secret of Kyoshiro and Kyo, she hopes to unravel their mystery (and possibly to collect on their bounty).

Izumono Okuni
A mysterious and powerful woman, Okuni is a former priestess of the Izumo temple who claims to have been Kyo's lover.

Jiban
A samurai who was shamed by Kyo at Sekigahara. He took a job as a yojimbo to a local Daikan in order to perfect his killing technique. Now he's ready to face Kyo again and regain his honor.

Kamijyo Akimine

Samurai Deeper Kyo is Akimine-san's first published manga. It began appearing in October of 1999 in Japan in Shuukan Shounen Magazine. The 18th volume was published in November, 2002 by Kodansha. It has also spawned a successful anime series from Studio Deen.